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Periodic Fever Syndromes Sequencing Panel

  • Summary and Pricing
  • Clinical Features and Genetics
  • Citations
  • Methods
  • Ordering/Specimens
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TEST METHODS

NGS Sequencing

Test Code Test Copy GenesCPT Code Copy CPT Codes
1931 CARD14 81479 Add to Order
ELANE 81479
IL36RN 81479
LPIN2 81479
MEFV 81404
MVK 81479
NLRP12 81479
NLRP3 81479
NOD2 81479
PSMB8 81479
PSTPIP1 81479
TNFAIP3 81479
TNFRSF1A 81479
Full Panel Price* $1440.00
Test Code Test Copy Genes Total Price CPT Codes Copy CPT Codes
1931 Genes x (13) $1440.00 81404, 81479(x12) Add to Order
Pricing Comment

If you would like to order a subset of these genes contact us to discuss pricing.

Targeted Testing

For ordering targeted known variants, please proceed to our Targeted Variants landing page.

Turnaround Time

The great majority of tests are completed within 28 days.

Clinical Sensitivity

Analytical sensitivity for detection of causative variants in the MEFV, MVK, NLRP3, NLRP12, TNFRSF1A, LPIN2, ELANE, and PSTPIP1 is >95% as gross deletions have not been reported. Clinical sensitivity is variable. In patients diagnosed with familial mediterranean fever, an identifiable pathogenic variant in the MEFV gene was found in 70-80% of cases (Aksentijevich et al. 1999). In patients with biochemical evidence of impaired mevalonate kinase activity and suspected Hyper-IgD syndrome or mevalonic aciduria, causative variants in the MVK gene were found ~95% of cases (Mandey et al. 2006; Bader-Meunier et al. 2011). In tumor necrosis factor receptor-associated periodic syndrome patients, causative variants in the TNFRSF1A gene are found in 40-50% of familial and ~5% of sporadic cases (Aksentijevich et al. 2001; Dode et al. 2002). Causative variants in NLRP3 have been identified in >85% of patients with familial cold autoinflammatory syndrome and ~60% of patients with chronic infantile neurological cutaneous articular syndrome (Aksentijevich et al. 2007; Aksentijevich et al. 2002). Clinical sensitivity for detection of ELANE causative variants in patients with cyclic neutropenia varies with two reports finding causative variants in 31 of 81 and 21 of 24 patients respectively (Dale et al. 2000; Bellanne-Chantelot et al. 2004). In an international registry for pediatric granulomatous diseases, 75 individuals were assessed for pathogenic variants in the NOD2 gene. In this cohort, 45 symptomatic patients had a pathogenic variant in the NOD2 gene. Of these 45 individuals, 22 were familial (Blau syndrome) and 23 were sporadic (Early Onset Sarcoidosis) form of PGA (Rosé et. al. 2009)

Clinical sensitivity cannot be estimated for pyogenic arthritis pyoderma gangrenosum and cystic acne syndrome, majeed syndrome, CANDLE, DITRA, TNFAIP3-mediated early onset autoinflammatory disease, CAMPS and NLRP12-associated autoinflammatory disorder because only a small number of cases have been reported.

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Deletion/Duplication Testing via aCGH

Test Code Test Copy GenesIndividual Gene PriceCPT Code Copy CPT Codes
600 CARD14$690.00 81479 Add to Order
ELANE$690.00 81479
IL36RN$690.00 81479
LPIN2$690.00 81479
MEFV$690.00 81479
MVK$690.00 81479
NLRP12$690.00 81479
NLRP3$690.00 81479
PSMB8$690.00 81479
PSTPIP1$690.00 81479
TNFRSF1A$690.00 81479
Full Panel Price* $1290.00
Test Code Test Copy Genes Total Price CPT Codes Copy CPT Codes
600 Genes x (11) $1290.00 81479(x11) Add to Order
Pricing Comment

# of Genes Ordered

Total Price

1

$690

2

$730

3

$770

4-10

$840

11-30

$1,290

31-100

$1,670

Over 100

Call for quote

Turnaround Time

The great majority of tests are completed within 28 days.

Clinical Sensitivity

Thus far, no gross deletions or duplications have been reported in the ELANE, CARD14, NOD2, PSMB8, NLRP3, NLRP12, PSTPIP1, LPIN2, TNFRSF1A, IL36RN, or MEFV genes (Human Gene Mutation Database). A gross deletion involving exon 1 of TNFAIP3 has been reported in one patient with Sjögren's syndrome (Nocturne et al. 2013). Deletions in the MVK gene have been reported in three cases, but account for less that 5% of all cases of mevalonate kinase deficiency (Takada et. al. 2003; Cuisset et. al. 2001; Zhang et. al. 2015).

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Clinical Features

Periodic Fever Syndromes (PFS) (also known as monogenic autoinflammatory syndromes) is a collective group of disorders highlighted by recurrent fever and inflammatory episodes. Fever episodes can range from hours up to ~ 2 weeks with recurrences ranging from several bouts per month to a few per year. PFS frequently involve inflammation of the skin, serous membranes, joints, lymph nodes, gastrointestinal tract, and nervous system. Onset of PFS occurs during the first year of life or early childhood with only familial mediterranean fever (FMF) and tumor necrosis factor receptor-associated periodic syndrome having cases of adult onset described. In severe cases, development of amyloidosis may occur. PFS are caused by aberrant inflammasome activation leading to heightened interleukin 1 levels and innate immune dysregulation. Differential diagnosis of individual PFS is important for employing appropriate therapeutics such as colchicine to treat FMF or antibiotics for cyclic neutropenia. PFS may mirror other immune dysfunction disorders such as cyclic neutropenia and SCID due to recurrent fever bouts, but unlike those disorders, bouts are not a result of recurrent infections (Caso et al. 2013; Touitou et al. 2013).

Disorders tested in this PFS panel include familial mediterranean fever/FMF, familial cold autoinflammatory syndrome/FCAS, muckle-wells syndrome/MWS, chronic infantile neurological cutaneous articular syndrome/CINCA, tumor necrosis factor receptor-associated periodic syndrome/TRAPS, mevalonate kinase deficiency/MKD, NLRP12 associated autoinflammatory disorder, pyogenic arthritis pyoderma gangrenosum and cystic acne syndrome/PAPA, majeed syndrome, cyclic neutropenia, pediatric granulomatous arthritis/PGA, CANDLE syndrome, DITRA syndrome, CARD14 mediated psoriasis/CAMPS, and TNFAIP3-mediated early onset autoinflammatory disease (Sanchez et al. 2013).

Genetics

Autosomal dominant PFS disorders include TRAPS (TNFRSF1A), FACS (NLRP3), MWS (NLRP3), CINCA (NLRP3), NLRP12 associated autoinflammatory disorder (NLRP12), PAPA (PSTPIP1), CAMPS (CARD14), TNFAIP3-mediated early onset inflammatory disease, PGA (NOD2), and cyclic neutropenia (ELANE). Autosomal recessive PFS disorders include FMF (MEFV), MKD (MVK), CANDLE (PSMB8), DITRA (IL36RN) and majeed syndrome (LPIN2) (Caso et al. 2013; Sanchez et al. 2013).

See individual test descriptions for additional information on the molecular biology of each gene product.

Testing Strategy

For this NGS test, the full coding regions plus ~20bp of non-coding DNA flanking each exon are sequenced for each of the genes listed below.  Sequencing is accomplished by capturing specific regions with an optimized solution-based hybridization method, followed by massively parallel sequencing of the captured DNA fragments. Additional Sanger sequencing is performed for any regions not captured or with insufficient number of sequence reads. All pathogenic, undocumented and questionable variant calls are confirmed by Sanger sequencing. 

This panel provides 100% coverage of the aforementioned regions of the indicated genes. We define coverage as > 20X NGS reads for exons and 0-10 bases of flanking DNA, > 10X NGS reads for 11-20 bases of flanking DNA, or Sanger sequencing.

Indications for Test

Candidates present with periodic/episodic fevers without an underlying infection typically within the first few years of life. Attacks are short and recurrent with spontaneous remission. Cyclic Neutropenia mirrors many features of other PFS, but is triggered by underlying infection. Fever episodes often present with elevated serum amyloid A, elevated erythrocyte sedimentation rate, and leukocytosis (Caso et al. 2013; Touitou et al. 2013).

Genes

Official Gene Symbol OMIM ID
CARD14 607211
ELANE 130130
IL36RN 605507
LPIN2 605519
MEFV 608107
MVK 251170
NLRP12 609648
NLRP3 606416
NOD2 605956
PSMB8 177046
PSTPIP1 606347
TNFAIP3 191163
TNFRSF1A 191190
Inheritance Abbreviation
Autosomal Dominant AD
Autosomal Recessive AR
X-Linked XL
Mitochondrial MT

Diseases

Name Inheritance OMIM ID
Autoinflammatory Syndrome, Familial, Behcet-like AD 616744
Chronic Atypical Neutrophilic Dermatosis with Lipodystrophy and Elevated Temperature Syndrome AR 256040
Chronic Infantile Neurological, Cutaneous And Articular Syndrome AD 607115
Familial Amyloid Nephropathy With Urticaria And Deafness AD 191900
Familial Cold Autoinflammatory Syndrome 2 AD 611762
Familial Mediterranean Fever AR 249100
Familial Mediterranean Fever, Autosomal Dominant AD 134610
Granulomatous Inflammatory Arthritis, Dermatitis, And Uveitis, Familial AD 186580
Hyperimmunoglobulin D With Periodic Fever AR 260920
Inflammatory Bowel Disease 1 266600
Interleukin 36 Receptor Antagonist Deficiency AR 614204
Majeed Syndrome AR 609628
Psoriasis susceptibility 2 AD 602723
Pyogenic Arthritis, Pyoderma Gangrenosum And Acne AD 604416
Severe Congenital Neutropenia Autosomal Dominant AD 202700
Tnf Receptor-Associated Periodic Fever Syndrome (Traps) AD 142680

Related Tests

Name
CANDLE Syndrome via the PSMB8 Gene
Cryopyrin-Associated Periodic Syndromes via the NLRP3 Gene
DITRA via the IL36RN Gene
Familial Cold Autoinflammatory Syndrome 2 via the NLRP12 Gene
Familial Mediterranean Fever (Renal Amyloidosis) via the MEFV Gene
Familial Pityriasis Rubra Pilaris (PRP) via the CARD14 Gene
Hyper IgD Syndrome/Mevalonate Aciduria via the MVK Gene
Majeed Syndrome via the LPIN2 Gene
Pediatric Granulomatous Arthritis via the NOD2 Gene
Severe Congenital Neutropenia and Cyclic Neutropenia via the ELANE Gene
Severe Congenital Neutropenia Sequencing Panel
TNF-Receptor Associated Periodic Syndrome (TRAPS) via the TNFRSF1A Gene

CONTACTS

Genetic Counselors
Geneticist
Citations
  • Aksentijevich I. et al. 1999. The American Journal of Human Genetics. 64: 949–962. PubMed ID: 10090880
  • Aksentijevich I. et al. 2001. American Journal of Human Genetics. 69: 301-14. PubMed ID: 11443543
  • Aksentijevich I. et al. 2002. Arthritis and Rheumatism. 46: 3340-8. PubMed ID: 12483741
  • Aksentijevich I. et al. 2007. Arthritis and Rheumatism. 56: 1273-85. PubMed ID: 17393462
  • Bader-Meunier B. et al. 2011. Pediatrics. 128: e152-e159. PubMed ID: 21708801
  • Bellanne-Chantelot C. 2004. Blood. 103: 4119-25. PubMed ID: 14962902
  • Caso F. et al. 2013. International Journal of Rheumatology. 2013: 513782. PubMed ID: 24282415
  • Cuisset L. et al. 2001. European Journal of Human Genetics. 9: 260-6. PubMed ID: 11313769
  • Dale David C. et al. 2000. Blood. 96: 2317–22. PubMed ID: 11001877
  • Dodé C. et al. 2002. Nephrology, Dialysis, Transplantation. 17: 1212-7. PubMed ID: 12105243
  • Human Gene Mutation Database (Bio-base).
  • Mandey Saskia H.L. et al. 2006. Human Mutation. 27: 796-802. PubMed ID: 16835861
  • Nocturne G. et al. 2013. Blood. 122: 4068-76. PubMed ID: 24159176
  • Rosé et. al. 2009. Arthritis Rheum. 2009. 60: 1797-803. PubMed ID: 19479837
  • Sanchez G.A. et al. 2013. Rheumatic Diseases Clinics of North America. 39: 701-34. PubMed ID: 24182851
  • Takada K. et al. 2003. Arthritis and Rheumatism. 48: 2645-51. PubMed ID: 13130485
  • Touitou I. et al. 2013. Orphanet Journal of Rare Diseases. 8: 162. PubMed ID: 24131530
  • Zhang Z. et al. 2015. Elife. 4: e06322. PubMed ID: 26202976
Order Kits
TEST METHODS

NextGen Sequencing using PG-Select Capture Probes

Test Procedure

We use a combination of Next Generation Sequencing (NGS) and Sanger sequencing technologies to cover the full coding regions of the listed genes plus ~20 bases of non-coding DNA flanking each exon.  As required, genomic DNA is extracted from the patient specimen.  For NGS, patient DNA corresponding to these regions is captured using an optimized set of DNA hybridization probes.  Captured DNA is sequenced using Illumina’s Reversible Dye Terminator (RDT) platform (Illumina, San Diego, CA, USA).  Regions with insufficient coverage by NGS are covered by Sanger sequencing.  All pathogenic, likely pathogenic, or variants of uncertain significance are confirmed by Sanger sequencing.

For Sanger sequencing, Polymerase Chain Reaction (PCR) is used to amplify targeted regions.  After purification of the PCR products, cycle sequencing is carried out using the ABI Big Dye Terminator v.3.0 kit.  PCR products are resolved by electrophoresis on an ABI 3730xl capillary sequencer.  In nearly all cases, cycle sequencing is performed separately in both the forward and reverse directions.

Patient DNA sequence is aligned to the genomic reference sequence for the indicated gene region(s). All differences from the reference sequences (sequence variants) are assigned to one of five interpretation categories, listed below, per ACMG Guidelines (Richards et al. 2015).

(1) Pathogenic Variants
(2) Likely Pathogenic Variants
(3) Variants of Uncertain Significance
(4) Likely Benign Variants
(5) Benign, Common Variants

Human Genome Variation Society (HGVS) recommendations are used to describe sequence variants (http://www.hgvs.org).  Rare variants and undocumented variants are nearly always classified as likely benign if there is no indication that they alter protein sequence or disrupt splicing.

Analytical Validity

As of March 2016, 6.36 Mb of sequence (83 genes, 1557 exons) generated in our lab was compared between Sanger and NextGen methodologies. We detected no differences between the two methods. The comparison involved 6400 total sequence variants (differences from the reference sequences). Of these, 6144 were nucleotide substitutions and 256 were insertions or deletions. About 65% of the variants were heterozygous and 35% homozygous. The insertions and deletions ranged in length from 1 to over 100 nucleotides.

In silico validation of insertions and deletions in 20 replicates of 5 genes was also performed. The validation included insertions and deletions of lengths between 1 and 100 nucleotides. Insertions tested in silico: 2200 between 1 and 5 nucleotides, 625 between 6 and 10 nucleotides, 29 between 11 and 20 nucleotides, 25 between 21 and 49 nucleotides, and 23 at or greater than 50 nucleotides, with the largest at 98 nucleotides. All insertions were detected. Deletions tested in silico: 1813 between 1 and 5 nucleotides, 97 between 6 and 10 nucleotides, 32 between 11 and 20 nucleotides, 20 between 21 and 49 nucleotides, and 39 at or greater than 50 nucleotides, with the largest at 96 nucleotides. All deletions less than 50 nucleotides in length were detected, 13 greater than 50 nucleotides in length were missed. Our standard NextGen sequence variant calling algorithms are generally not capable of detecting insertions (duplications) or heterozygous deletions greater than 100 nucleotides. Large homozygous deletions appear to be detectable.   

Analytical Limitations

Interpretation of the test results is limited by the information that is currently available.  Better interpretation should be possible in the future as more data and knowledge about human genetics and this specific disorder are accumulated.

When Sanger sequencing does not reveal any difference from the reference sequence, or when a sequence variant is homozygous, we cannot be certain that we were able to detect both patient alleles.  Occasionally, a patient may carry an allele which does not amplify, due to a large deletion or insertion.   In these cases, the report will contain no information about the second allele.  Our Sanger and NGS Sequencing tests are generally not capable of detecting Copy Number Variants (CNVs).

We sequence all coding exons for each given transcript, plus ~20 bp of flanking non-coding DNA for each exon.  Test reports contain no information about other portions of the gene, such as regulatory domains, deep intronic regions or any currently uncharacterized alternative exons.

In most cases, we are unable to determine the phase of sequence variants.  In particular, when we find two likely causative mutations for recessive disorders, we cannot be certain that the mutations are on different alleles.

Our ability to detect minor sequence variants due to somatic mosaicism is limited.  Sequence variants that are present in less than 50% of the patient’s nucleated cells may not be detected.

Runs of mononucleotide repeats (eg (A)n or (T)n) with n >8 in the reference sequence are generally not analyzed because of strand slippage during PCR.

Unless otherwise indicated, DNA sequence data is obtained from a specific cell-type (usually leukocytes from whole blood).   Test reports contain no information about the DNA sequence in other cell-types.

We cannot be certain that the reference sequences are correct.

Rare, low probability interpretations of sequencing results, such as for example the occurrence of de novo mutations in recessive disorders, are generally not included in the reports.

We have confidence in our ability to track a specimen once it has been received by PreventionGenetics.  However, we take no responsibility for any specimen labeling errors that occur before the sample arrives at PreventionGenetics.

Deletion/Duplication Testing Via Array Comparative Genomic Hybridization

Test Procedure

Equal amounts of genomic DNA from the patient and a gender matched reference sample are amplified and labeled with Cy3 and Cy5 dyes, respectively. To prevent any sample cross contamination, a unique sample tracking control is added into each patient sample. Each labeled patient product is then purified, quantified, and combined with the same amount of reference product. The combined sample is loaded onto the designed array and hybridized for at least 22-42 hours at 65°C. Arrays are then washed and scanned immediately with 2.5 µM resolution. Only data for the gene(s) of interest for each patient are extracted and analyzed.

Analytical Validity

PreventionGenetics' high density gene-centric custom designed aCGH enables the detection of relatively small deletions and duplications within a single exon of a given gene or deletions and duplications encompassing the entire gene. PreventionGenetics has established and verified this test's accuracy and precision.

Analytical Limitations

Our dense probe coverage may allow detection of deletions/duplications down to 100 bp; however due to limitations and probe spacing this cannot be guaranteed across all exons of all genes. Therefore, some copy number changes smaller than 100-300 bp within a targeted large exon may not be detected by our array.

This array may not detect deletions and duplications present at low levels of mosaicism or those present in genes that have pseudogene copies or repeats elsewhere in the genome.

aCGH will not detect balanced translocations, inversions, or point mutations that may be responsible for the clinical phenotype.

Breakpoints, if occurring outside the targeted gene, may be hard to define.

The sensitivity of this assay may be reduced when DNA is extracted by an outside laboratory.

Order Kits

Ordering Options


myPrevent - Online Ordering
  • The test can be added to your online orders in the Summary and Pricing section.
  • Once the test has been added log in to myPrevent to fill out an online requisition form.
REQUISITION FORM
  • A completed requisition form must accompany all specimens.
  • Billing information along with specimen and shipping instructions are within the requisition form.
  • All testing must be ordered by a qualified healthcare provider.

SPECIMEN TYPES
WHOLE BLOOD

(Delivery accepted Monday - Saturday)

  • Collect 3 ml -5 ml (5 ml preferred) of whole blood in EDTA (purple top tube) or ACD (yellow top tube). For Test #500-DNA Banking only, collect 10 ml -20 ml of whole blood.
  • For small babies, we require a minimum of 1 ml of blood.
  • Only one blood tube is required for multiple tests.
  • Ship blood tubes at room temperature in an insulated container. Do not freeze blood.
  • During hot weather, include a frozen ice pack in the shipping container. Place a paper towel or other thin material between the ice pack and the blood tube.
  • In cold weather, include an unfrozen ice pack in the shipping container as insulation.
  • At room temperature, blood specimen is stable for up to 48 hours.
  • If refrigerated, blood specimen is stable for up to one week.
  • Label the tube with the patient name, date of birth and/or ID number.

DNA

(Delivery accepted Monday - Saturday)

  • Send in screw cap tube at least 5 µg -10 µg of purified DNA at a concentration of at least 20 µg/ml for NGS and Sanger tests and at least 5 µg of purified DNA at a concentration of at least 100 µg/ml for gene-centric aCGH, MLPA, and CMA tests, minimum 2 µg for limited specimens.
  • For requests requiring more than one test, send an additional 5 µg DNA per test ordered when possible.
  • DNA may be shipped at room temperature.
  • Label the tube with the composition of the solute, DNA concentration as well as the patient’s name, date of birth, and/or ID number.
  • We only accept genomic DNA for testing. We do NOT accept products of whole genome amplification reactions or other amplification reactions.

CELL CULTURE

(Delivery preferred Monday - Thursday)

  • PreventionGenetics should be notified in advance of arrival of a cell culture.
  • Culture and send at least two T25 flasks of confluent cells.
  • Some panels may require additional flasks (dependent on size of genes, amount of Sanger sequencing required, etc.). Multiple test requests may also require additional flasks. Please contact us for details.
  • Send specimens in insulated, shatterproof container overnight.
  • Cell cultures may be shipped at room temperature or refrigerated.
  • Label the flasks with the patient name, date of birth, and/or ID number.
  • We strongly recommend maintaining a local back-up culture. We do not culture cells.
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