Specimens & Shipping

Specimen Requirements

Acceptable Specimen Types

Specimen Requirement Details

Please contact us with additional specimen requirement questions.

Whole Blood: Collect 3 ml - 5 ml of whole blood in EDTA (purple top tube) or ACD (yellow top tube), minimum 1 ml for small infants. Heparin (green top tube) is strongly discouraged.

DNA: Send in a screw cap tube at least 5 µg -10 µg of purified DNA at a concentration of at least 100 ng/uL, minimum 2 µg for limited specimens. Indicate concentration on tube label. For requests requiring more than one test, send an additional 5 µg DNA per test ordered when possible. For rapid tests, good DNA quality is of utmost importance.

Fetal (CVS/AMNIOCYTES) and other cell cultures: Culture and send at least two, T25 flasks of confluent cells. For sequencing or gene-centric aCGH panels, two flasks are often sufficient; however, some panels may require additional flasks (dependent on size of genes, amount of Sanger sequencing required, etc.). Multiple test requests may also require additional flasks. Please contact us for details. We strongly recommend maintaining a back-up culture.

Fetal cell cultures are available at PreventionGenetics from direct amniotic fluid, chorionic villi, products of conception (POC), or other tissue via Test Code #995. Collect 10 ml - 20 ml of direct amniotic fluid or 5 mg - 10 mg cleaned CVS tissue (~15-20 cleaned villi) or 2mm x 2mm x 2mm fresh tissue. CPT code 88235 for amniotic fluid/chorionic villi or 88233 for POC or tissue specimens.

Fresh, Frozen Tissue: Collect 2mm x 2mm x 2mm tissue and flash freeze. Tissue to be sent frozen (preferably dry ice). If cell culture is desired, tissue should not be frozen. Contact us for additional details.

Saliva: Oragene™ or GeneFiX™ Saliva Collection kit used according to manufacturer instructions.

Saliva collection instructions are available in 9 languages, including English. To request special instructions for patients, add a note in the Comments section of the kit order indicating which language is needed. Additional languages available include: Chinese, French, German, Mayotte (French), Portuguese, Spanish (Mexican), Spanish (Spain), Swedish

Additional instructions to help families collecting samples at home are included in each home saliva kit order.

OCD-100 Buccal Swab: OCD-100 Buccal Swab used according to manufacturer instructions. Buccal swabs are most appropriate for targeted, known variant testing.

Saliva Kit Ordering Ordering Instructions:


Direct Amniotic Fluid / Chorionic Villi: Collect 10 ml -20 ml of direct amniotic fluid or 5 mg -10 mg cleaned CVS tissue (~15-20 cleaned villi). A back-up culture is required for NGS. If there is no indication a back-up culture is set-up elsewhere, we will start one at PreventionGenetics. We strongly recommend maintaining a back-up culture for any other testing on direct prenatal specimens. Fetal cell culturing is available (Test Code #995).

Shipping & Handling Instructions

Mailing Address
PreventionGenetics - Diagnostic Lab
3800 S. Business Park Ave.
Marshfield, WI 54449 USA

Testing Kits
Clinical testing kits with prepaid return shipping are available for U.S. and Canadian clients. Kits are provided to International clients without the return postage. Submit requests through our website or contact our Client Service Representatives at (715) 387-0484, ext. 0.

Label all specimen containers with the patient's name, date of birth, and/or ID number. At least two identifiers should be listed on specimen containers. Specimen deliveries are accepted Monday-Saturday for all specimen types. However, urgent and/or sensitive specimens, such as cell cultures, direct amniotic fluid, or direct chorionic villi, are preferred to arrive MondayThursday. Urgent / sensitive specimens shipped on Thursday should be marked for overnight delivery; those sent Friday should be marked for overnight and Saturday delivery. Contact us to make arrangements. Holiday schedules will be posted on our website at least one week prior to major holidays.

DO NOT FREEZE. During hot weather, include a frozen ice pack in the shipping container. Place a paper towel or other thin material between the ice pack and the blood tube. In cold weather include an unfrozen ice pack in the shipping container as insulation. At room temperature, blood specimen is stable for up to 48 hours. If refrigerated, blood specimen is stable for up to one week.

Specimens may be shipped at room temperature. For DNA, label the tube with the composition of the solute and DNA concentration along with the patient's name, date of birth, and/or ID number. We only accept genomic DNA for testing; we do not accept products of whole genome amplification reactions or other amplification reactions. DNA must be extracted from a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by CAP and/or CMS.

Send specimens overnight in an insulated, shatterproof container. Direct AF/CVS, POC, or tissue specimens can be sent in saline or culture media at room temperature for culturing at PreventionGenetics.

Please sign the Prenatal Healthcare Provider's Statement for ongoing pregnancies and contact us in advance regarding prenatal test requests. When possible, ship prenatal samples to arrive at PreventionGenetics no later than Thursday.

For quality control purposes, the Prevention Genetics DNA Genotyping Panel is performed on all clinical specimens. Genotyping results are not included in test reports.

DNA Banking has a reduced price of $98 for patients if clinical testing is also being performed at PreventionGenetics. Visit our website at www.PreventionGenetics.com/pgdnabank for more information. Questions about PGDNABanking, contact us at (715) 387-0484, ext. 151, or email: dnabanking@preventiongenetics.com.

For additional questions or concerns, contact a Client Service Representatives at (715) 387-0484, ext. 0, or our Genetic Counseling Team at option 2, or email: support@preventiongenetics.com.